齐炳理1，逯 刚1*，董立雅1，唐亚娟1，包倩倩1，郝 晖1，宋晓东2，程聪洋2
（1 内蒙古蒙牛乳业（集团）股份有限公司，内蒙古 呼和浩特 011500；
2 国家市场监督管理总局重点实验室（乳品质量数智监控技术），内蒙古 呼和浩特 011500）
摘 要：目的评估9株乳酸菌对HT-29细胞的粘附能力。方法以HT-29细胞为评价模型，采用CFDA-SE标记 细菌，标记后的细菌与HT-29细胞共孵育2h，采用多功能酶标仪测定荧光强度，计算细菌粘附率。结果9株乳 酸菌的粘附率结果显示，总体粘附率比较，动物双歧杆菌乳亚种（Bifidobacteriumanimalissubsp.lactis）>干酪乳酪 杆菌（Lacticaseibacillus casei）>植物乳植杆菌（Lactiplantibacillus plantarum）>鼠李糖乳酪杆菌（Lacticaseibacillus rhamnosus）>副干酪乳酪杆菌（Lacticaseibacillus paracasei）；动物双歧杆菌乳亚种-1 的粘附率最高，为 8.08%；干 酪乳酪杆菌-4 粘附率次之，为6.70%；副干酪乳酪杆菌-9 的粘附率最低，为1.05%；动物双歧杆菌乳亚种-1的 粘附率显著高于动物双歧杆菌乳亚种-2（P<0.05）和动物双歧杆菌乳亚种-3（P<0.05）；植物乳植杆菌-5 的粘附率显 著高于植物乳植杆菌-6（P<0.05）。结论乳酸菌的粘附能力具有种属特异性，不同种属粘附能力不同，同种不同菌 株粘附能力也不同，其中动物双歧杆菌乳亚种及干酪乳酪杆菌的粘附能力较高。
关键词：乳酸菌；HT-29 细胞；粘附能力
Study on Adhesion Ability of 9 Lactic Acid Bacteria to HT-29 Cells
QI Bing-li1，LU Gang1*，DONG Li-ya1，TANG Ya-juan1，BAO Qian-qian1，HAO Hui1，SONG Xiao-dong2， CHENG Cong-yang2
（1 Inner Mongolia Mengniu Dairy（group）Co.，Ltd.，Hohhot 011500，China；
2 Key Laboratory of Dairy Quality Digital Intelligence Monitoring Technology，State Administration for Market Regulation，Hohhot 011500，China）
Abstract: [Objective] Evaluating the adhesion ability of 9 strains of lactic acid bacteria to HT-29 cells. [Methods] HT 29 cells were used as the evaluation model，and CFDA-SE was used to label the bacteria. The labeled bacteria were then co-incubated with the HT-29 cells for two hours，after which the fluorescence intensity was measured using a microplate reader. The bacterial adhesion rate was then calculated. [Results] The adhesion rates of the 9 lactic acid bacteria strains were compared. Overall，the order of adhesion rates was as follows：Bifidobacterium animalis subsp. lactis > Lacticaseibacillus casei > Lactiplantibacillus plantarum > Lacticaseibacillus rhamnosus > Lacticaseibacillus paracasei. Bifidobacterium animalis subsp. lactis-1 had the highest adhesion rate of 8.08%. Lacticaseibacillus casei-4 had the second highest adhesion rate of 6.70%，and Lacticaseibacillus paracasei-9 had the lowest adhesion rate of 1.05% . Bifidobacterium animalis subsp. Lactis- 1 had a significantly higher adhesion rate than Bifidobacterium animalis subsp. Lactis- 2 （P<0.05）and Bifidobacterium animalis subsp. Lactis- 3 （P<0.05）. Lactiplantibacillus plantarum-5 had a significantly higher adhesion rate than Lactiplantibacillus plantarum 6（P<0.05）. [Conclusion] The adhesion ability of lactic acid bacteria is species- specific. Different species and strains have different adhesion abilities. Among them，Bifidobacterium animalis subsp. lactis and Lacticaseibacillus casei have higher adhesion abilities than others.
Key words: lactic acid bacteria；HT-29 cell；adhesion ability
中图分类号：TS201.3 文献标志码：A 文章编号：1007-7871（2026）04-0009-05
2026，Vol.29，No.4