贝荣廷1,2,3，何焜鹏1,2,3，黄 韡1,2,3，韦 誉1,2,3，利 通1,2,3，林 丹1,2,3，郑泽洋1,2,3，庄俊钰1,2,3*
（1 广东省食品工业研究所有限公司，广东 广州 511442；
2 广东省食品工业公共实验室，广东 广州 511442；
3 广东食品质量监督检验站，广东 广州 511442）
摘 要：建立了一种食品中磷脂酰丝氨酸测定的正相高效液相色谱法。方法：试样中磷脂酰丝氨酸经提取净化后， 借助蒸发光散射检测器（ELSD）进行检测，外标法定量。结果：在20μg/mL～300μg/mL 范围内，线性相关系数大于 0.999，方法检出限为 6mg/100g，定量限定为 20mg/100g；在低、中、高 3 个添加水平下的回收率在 84.32%～ 107.9%，相对标准偏差在 1.2%～6.6%。结论：该方法方便、快捷、准确、经济，可应用于监管部门对食品中磷脂酰丝 氨酸监管，以更好地规范食品企业的市场行为，保护消费者和企业双方的利益。
关键词：食品；磷脂酰丝氨酸；正相高效液相色谱；固相萃取
Determination of Phosphatidylserine in Food
BEI Rong-ting1,2,3，HE Kun-peng1,2,3，HUANG WEI1,2,3，WEI Yu1,2,3，LI Tong1,2,3，LIN Dan1,2,3，ZHENG Ze-yang1,2,3， ZHUANG Jun-yu1,2,3*
（1 Guangdong Food Industry Institute Co.，Ltd.，Guangzhou 511442，China；
2 Guangdong Provincial Public Laboratory of Food Industry，Guangzhou 511442，China；
3 Guangdong Food Quality Supervision and Inspection Station，Guangzhou 511442，China）
Abstract: Objective：A positive- phase high- performance liquid chromatography（HPLC） method for determining phosphatidylserine in food has been established. Methods：The phosphatidylserine in the sample was extracted and purified，and then detected by evaporative light scattering detector（ELSD），with external standard method for quantification. Results：Within the concentration range of 20μg/mL～300μg/mL，the correlation coefficient of linear regression was greater than 0.999，with an analytical detection limit of 6mg/100g and a quantitative limit of 20mg/ 100g. The recovery rates were 84.32%～107.9% at three added levels（low，middle，high）with standard deviations between 1.2% to 6.6%. Conclusion：This method is convenient，quick，accurate，economical，and it can be applied to the supervision of phosphatidylserine in food by government regulatory departments，so as to better regulate the market behavior of food enterprises and protect the interests of both consumers and enterprises.
Key words: food；phosphatidylserine；positive-phase high-performance liquid chromatography；evaporative light-scattering detector
中图分类号：TS207.3 文献标志码：A 文章编号：1007-7871（2026）04-0019-07
2026，Vol.29，No.4